Rabbit Anti-NeuN
（Neuronal Nuclei）
Catalog Number : RS-1613R
Quantity size : 0.1ml （dilute with pH 7.4 0.01 M PBS or antibody diluent ）
Background: Vertebrate neuron-specific nuclear protein called NeuN （Neuronal Nuclei） is an excellent marker for neurons in primary cultures and in retinoic acid-stimulated P19 cells. It is also useful for identifying neurons in transplants. NeuN is a neuron-specific, DNA-binding nuclear protein in vertebrates. In mice, NeuN is observed in most neuronal cell types throughout the nervous system, including cerebellum, cerebral cortex, hippocampus, thalamus and spinal cord, as well as the dorsal root ganglia, sympathetic chain ganglia and enteric ganglia of the peripheral nervous system. NeuN immunoreactivity is first observed in neurons when they become post-mitotic and are initiating cellular and morphological differentiation. No staining is observed in proliferative zones. NeuN has been used as an immunohistochemical marker for excitotoxic lesions of the brain as well as in the diagnosis of a wide range of human tissue specimens from the central and peripheral nervous systems.
Specificity : •anti-NeuN is a rabbit polyclonal antibody unconjugated
•specific for NeuN of human, mo, rat
•epitope mapping near the amino terminus of NeuN
of human, mo, rat origin
•use for western blotting, elisa, immunoprecipitation
and immunohistochemistry
• Protein A affinity chromatography purification, purity :>95%
• Isotype: IgG
Application : • Western blotting: 1:100-500
• Immunohistochemistry: 1:100-500
•ELISA: 1:500-1000
•Optimal working dilutions must be determined by the end user.
Storage: Store at –20 ºC for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20ºC. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody, the antibody is stable for at least two weeks at 2-4 ºC.
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
